Vineyard soils show an increased risk of degradation due to being intensively cultivated. The preservation of soil integrity and fertility is a key concept of organic and biodynamic farming. However, both systems are also subject to criticism due to their higher amount of plant protection products used and their increased traffic intensity compared to integrated viticulture, both detrimental to soil quality. The aim of this study was therefore to assess long-term effects of these three management systems on chemical and physical soil quality parameters. For this purpose, topsoil samples were taken in a long-term field trial vineyard at different positions and examined for bulk density, available water capacity (AWC), soil organic carbon (SOC), N, pH, and for total and bioavailable copper (Cu) concentrations. Biodynamic plots had a lower bulk density and higher SOC concentration than the integrated ones, which is probably due to the species-rich cover crop mixture used in the inter-row. However, organic and biodynamic farming showed an accumulation of copper in the under-vine area and in the tractor track, which is problematic for soil fertility in the long-term. Therefore, alternatives for copper in plant protection are necessary to ensure sustainable soil quality through organic and biodynamic viticulture.